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· Sleek internal design as well as large vertical twin membranes for ultra fast concentration
· High retentate recovery > 95 %
· pH 1-14 suited polystyrene housing with high chemical compatibility
· High flux PES membrane
· Universal rotor compatibility
· Safe sample concentration and easy recovery due to unique, angular and pipette friendly dead-stop pocket
· Volume graduation for easy detection / monitoring of the concentrate volume
· > 100-fold concentration with twin vertical membranes
描述
Vivaspin® Turbo 15 can handle up to 15 ml sample volume in swing bucket rotors and 11ml in fixed angle rotors accepting 50 ml centrifuge tubes.
The Vivaspin® Turbo 15 optimised design, the sleek internal profile, ensures maximum process speeds right the way down to the last few micro litres after > 100 fold concentration.
The UV joining technology allows for a smooth joint transition between membrane and plastic housing, allowing the collection of the complete concentrated sample into the unique pipette friendly dead stop pocket.
Rotor Compatibility
Please note: Vivaspin® Turbo 15 (30 mm x 118 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes. These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 or Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin® 15, which can be used in either round bottom or conical centrifuge tubes.
Performance Characteristics
Time to concentrate up to 20x [min.] at | ||||
Rotor | Swing Bucket | 25° Fixed angle | ||
Centrifugal speed | 4,000 g | 4,000 g | ||
Start volume | 15 ml | 9 ml | ||
Min. | Rec. | Min. | Rec. | |
Cytochrome (0.25 mg/ml) (12,400 MW) | ||||
5 MWCO PES | 30 | 98 % | 50 | 98 % |
Lysozyme (0.25 mg/ml) (14,300 MW) | ||||
5 MWCO PES | 33 | 96 % | 50 | 96 % |
a-Chymotrypsin (1 mg/ml) (25,000 MW) | ||||
10 MWCO PES | 10 | 95 % | 10 | 95 % |
BSA (1 mg/ml) (66,000 MW) | ||||
10 MWCO PES | 10 | 99 % | 10 | 99 % |
30 MWCO PES | 8 | 98 % | 10 | 98 % |
IgG (1 mg/ml) (160,000 MW) | ||||
30 MWCO PES | 23 | 95 % | 17 | 95 % |
50 MWCO PES | 20 | 94 % | 15 | 94 % |
技术规格：
Dimensions
宽 | 30 mm |
总长度 | 118 cm |
有效膜面积 | 7,2 cm² |
残留体积 | 10 µl |
死体积 | 60 / 100 µl |
Materials of Construction
集中器盖 | 聚苯乙烯 |
膜 | 聚醚砜(PES) |
一般属性
主体 | 丁二烯-聚苯乙烯共聚物 |
滤液容器 | 聚丙烯(PP) |
水平转头离心力 | 4.000 x g |
角转头离心力 | 4.000 x g |
MWCO | 30.000 Dalton |
类型 | 离心式机组 |
容量范围 | 4 - 15 ml |
证书 | 无 |
市场 | 生物制药; 研究 |
应用 | 浓缩; 脱盐|缓冲液更换 |
样品类型 | 纳米微粒; 蛋白; 病毒 |
超滤设备类型 | 离心超滤设备 |
超滤离心管、超滤管、浓缩超滤离心管、高速超滤离心管、超滤浓缩离心管、超滤浓缩装置
品牌：密理博Millipore、赛多利斯sartorius、美国Pall
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