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参数
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- 爱尔兰品牌
- JKY/K-HYT库号：M316051 型号
北京 海淀区
11天内发货
144台
产品详情
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植酸/总磷检测试剂盒(hytic&nbs;Acid&nbs;/&nbs;Total&nbs;hoshorus&nbs;Assay&nbs;Kit)&nbs;50次&nbs;爱尔兰&nbs;型号:JKY/K-HYT
库号：M316051&nbs;&nbs;&nbs;
名称：植酸/总磷检测试剂盒&nbs;
产品货号：K-HYT&nbs;
规格：50&nbs;
产地：爱尔兰&nbs;
数：&nbs;
英文名：hytic&nbs;Acid/Total&nbs;hoshorus&nbs;Assay&nbs;Kit
保存条件：4-6摄氏度
保期：1年以上
外观四个玻璃小瓶和两个60毫升聚丙烯瓶
比重&nbs;
在水中的溶解度易溶
H值&nbs;7.0
气味&nbs;无
粉末和液体形式
性&nbs;&nbs;十年或以上(冷冻机)
成分:
名称&nbs;比例
钠缓冲液60毫升&nbs;一小瓶（聚丙烯）
甘氨酸缓冲液&nbs;一瓶（聚丙烯）
植酸酶悬浮液&nbs;一个七毫升小瓶（玻璃）
碱性磷酸酶悬浮液&nbs;一个七毫升小瓶（玻璃）
磷标准溶液25毫升&nbs;一小瓶（玻璃）
燕麦粉的液&nbs;一毫升小瓶（玻璃）
试剂盒：
试剂盒适执行50个化验从
包含检测方法，另：
瓶1：钠缓冲液（25毫升，200毫米，H值5.5）和
叠氮化钠（0.02％瓦/视频作为剂）。
在4&nbs;°&nbs;C的“2年
瓶2：植酸酶暂停（1.2毫升，12000单位/毫升）。
在4&nbs;°&nbs;C的“2年
瓶3：甘氨酸缓冲液（25毫升，400毫米，H值10.4），化镁
（4毫米），锌（0.4毫米）和叠氮化钠
（0.02％瓦/&nbs;5）作为剂。
在4&nbs;°&nbs;C的“2年
瓶4：暂停碱性磷酸酶（1.2毫升，80单位/毫升）。
在4&nbs;°&nbs;C的“2年
瓶5：磷标准溶液（24毫升，50微克/毫升）。
和叠氮化钠（0.02％瓦/视频作为剂）。
在4&nbs;°&nbs;C的“2年
瓶6：燕麦粉粉（5克，磷含，
见瓶）。
“在室温下5年。
hytic&nbs;Acid&nbs;/&nbs;Total&nbs;hoshorus
A&nbs;simle&nbs;method&nbs;for&nbs;the&nbs;measurement&nbs;of&nbs;hytic&nbs;acid&nbs;/&nbs;total&nbs;hoshorus&nbs;in&nbs;food&nbs;and&nbs;feed&nbs;samles.&nbs;This&nbs;method&nbs;does&nbs;not&nbs;require&nbs;urification&nbs;of&nbs;hytic&nbs;acid&nbs;via&nbs;anion-exchange&nbs;chromatoahy&nbs;making&nbs;it&nbs;amenable&nbs;to&nbs;high&nbs;numbers&nbs;of&nbs;samles.
Catalogue&nbs;Number:&nbs;K-HYT
Content:&nbs;50&nbs;assays
Aearance&nbs;Four&nbs;glass&nbs;vials&nbs;and&nbs;two&nbs;60&nbs;mL&nbs;olyroylene&nbs;containers.
Secific&nbs;avity&nbs;Not&nbs;alicable
Solubility&nbs;in&nbs;Water&nbs;Readily&nbs;soluble.
H&nbs;Value&nbs;7.0
Odour&nbs;none
Form&nbs;owders&nbs;and&nbs;liquids
Stability&nbs;stable&nbs;in&nbs;a&nbs;freezer&nbs;for&nbs;ten&nbs;or&nbs;more&nbs;years
Inedients&nbs;Name&nbs;CAS&nbs;roortion
Sodium&nbs;acetate&nbs;buffer&nbs;One&nbs;60&nbs;ml&nbs;vial&nbs;(olyroylene)
Glycine&nbs;Buffer&nbs;One&nbs;container&nbs;(olyroylene)
hytase&nbs;susension&nbs;One&nbs;7&nbs;ml&nbs;vial&nbs;(glass)
Alkaline&nbs;hoshatase&nbs;susension&nbs;One&nbs;7&nbs;ml&nbs;vial&nbs;(glass)
hoshorus&nbs;standard&nbs;solution&nbs;One&nbs;25&nbs;ml&nbs;vial&nbs;(glass)
Oat&nbs;flour&nbs;control&nbs;One&nbs;12&nbs;ml&nbs;vial&nbs;(glass)
KITS:
Kits&nbs;suitable&nbs;for&nbs;erforming&nbs;50&nbs;assays&nbs;are&nbs;available.The
kits&nbs;contain&nbs;the&nbs;full&nbs;assay&nbs;method&nbs;lus:
Bottle&nbs;1:&nbs;Sodium&nbs;acetate&nbs;buffer&nbs;(25&nbs;mL,&nbs;200&nbs;mM,&nbs;H&nbs;5.5)&nbs;and
sodium&nbs;azide&nbs;(0.02&nbs;%&nbs;w/v)&nbs;as&nbs;a&nbs;reservative.
Stable&nbs;for&nbs;>&nbs;2&nbs;years&nbs;at&nbs;4°C.
Bottle&nbs;2:&nbs;hytase&nbs;susension&nbs;(1.2&nbs;mL,&nbs;12,000&nbs;U/mL).
Stable&nbs;for&nbs;>&nbs;2&nbs;years&nbs;at&nbs;4°C.
Bottle&nbs;3:&nbs;Glycine&nbs;buffer&nbs;(25&nbs;mL,&nbs;400&nbs;mM,&nbs;H&nbs;10.4),&nbs;lus&nbs;MgCl2
(4&nbs;mM),&nbs;ZnSO4&nbs;(0.4&nbs;mM)&nbs;and&nbs;sodium&nbs;azide
(0.02&nbs;%&nbs;w/v)&nbs;as&nbs;a&nbs;reservative.
Stable&nbs;for&nbs;>&nbs;2&nbs;years&nbs;at&nbs;4°C.
Bottle&nbs;4:&nbs;Alkaline&nbs;hoshatase&nbs;susension&nbs;(1.2&nbs;mL,&nbs;80&nbs;U/mL).
Stable&nbs;for&nbs;>&nbs;2&nbs;years&nbs;at&nbs;4°C.
Bottle&nbs;5:&nbs;hoshorus&nbs;standard&nbs;solution&nbs;(24&nbs;mL,&nbs;50&nbs;μg/mL).
and&nbs;sodium&nbs;azide&nbs;(0.02&nbs;%&nbs;w/v)&nbs;as&nbs;a&nbs;reservative.
Stable&nbs;for&nbs;>&nbs;2&nbs;years&nbs;at&nbs;4°C.
Bottle&nbs;6:&nbs;Oat&nbs;flour&nbs;control&nbs;owder&nbs;(5&nbs;g;&nbs;hoshorus&nbs;content,
see&nbs;bottle).
Stable&nbs;for&nbs;>&nbs;5&nbs;years&nbs;at&nbs;room&nbs;temerature.
INTRODUCTION:
hytic&nbs;acid&nbs;(hytate;&nbs;myo-inositol&nbs;1,2,3,4,5,6,&nbs;hexakishoshate)&nbs;is&nbs;the
rimary&nbs;source&nbs;of&nbs;inositol&nbs;and&nbs;storage&nbs;hoshorus&nbs;in&nbs;lant&nbs;seeds
contributing&nbs;~70&nbs;%&nbs;of&nbs;total&nbs;hoshorus.&nbs;The&nbs;abundance&nbs;of&nbs;hytic&nbs;acid
in&nbs;cereal&nbs;ains&nbs;is&nbs;a&nbs;concern&nbs;in&nbs;the&nbs;foods&nbs;and&nbs;animal&nbs;feeds&nbs;industries
because&nbs;the&nbs;hoshorus&nbs;in&nbs;this&nbs;form&nbs;is&nbs;unavailable&nbs;to&nbs;monogastric
animals&nbs;due&nbs;to&nbs;a&nbs;lack&nbs;of&nbs;endogenous&nbs;hytases;&nbs;enzymes&nbs;secific&nbs;for
the&nbs;dehoshorylation&nbs;of&nbs;hytic&nbs;acid.&nbs;In&nbs;addition,&nbs;the&nbs;strong&nbs;chelating
characteristic&nbs;of&nbs;hytic&nbs;acid&nbs;reduces&nbs;the&nbs;bioavailability&nbs;of&nbs;other
essential&nbs;dietary&nbs;nutrients&nbs;such&nbs;as&nbs;minerals&nbs;(e.g.&nbs;Ca2+,&nbs;Zn2+,&nbs;Mg2+,
Mn2+,&nbs;Fe2+/3+),&nbs;roteins&nbs;and&nbs;amino&nbs;acids.1&nbs;High&nbs;hytic&nbs;acid&nbs;content
feeds&nbs;are&nbs;generally&nbs;sulemented&nbs;with&nbs;inorganic&nbs;hoshate,&nbs;however
this&nbs;causes&nbs;increased&nbs;faecal&nbs;hoshate&nbs;levels&nbs;and&nbs;subsequent
eutrohication&nbs;of&nbs;waterways.&nbs;Alternatively,&nbs;sulementation&nbs;with
mercial&nbs;hytases&nbs;is&nbs;being&nbs;increasingly&nbs;oular&nbs;and&nbs;reduces&nbs;the
requirement&nbs;for&nbs;inorganic&nbs;hoshate&nbs;sulementation&nbs;as&nbs;well&nbs;as&nbs;the
associated&nbs;environmental&nbs;issues.
Currently,&nbs;there&nbs;is&nbs;no&nbs;mercially&nbs;available,&nbs;simle,&nbs;quantitative
method&nbs;for&nbs;hytic&nbs;acid&nbs;and&nbs;while&nbs;such&nbs;measurement&nbs;is&nbs;relatively
lex&nbs;the&nbs;generally&nbs;acceted&nbs;AOAC&nbs;Method&nbs;986.11&nbs;has
limitations.2&nbs;For&nbs;each&nbs;individual&nbs;analysis&nbs;the&nbs;method&nbs;requires
cumbersome&nbs;anion-exchange&nbs;urification&nbs;and&nbs;a&nbs;major&nbs;inherent
assumtion&nbs;here&nbs;is&nbs;that&nbs;only&nbs;hytic&nbs;acid&nbs;is&nbs;urified.&nbs;While&nbs;this
assumtion&nbs;is&nbs;viable&nbs;for&nbs;non-rocessed&nbs;ains&nbs;for&nbs;which&nbs;hytic&nbs;acid
rises&nbs;at&nbs;least&nbs;97&nbs;%&nbs;of&nbs;total&nbs;inositol&nbs;hoshates,&nbs;it&nbs;is&nbs;not&nbs;viable&nbs;for
rocessed&nbs;foods&nbs;and&nbs;feeds&nbs;which&nbs;can&nbs;contain&nbs;higher&nbs;levels&nbs;of&nbs;some
lower&nbs;myo-inositol&nbs;hoshate&nbs;forms&nbs;(i.e.&nbs;I3,&nbs;I4,&nbs;I5)&nbs;that&nbs;would&nbs;coelute
with&nbs;hytic&nbs;acid&nbs;and&nbs;contribute&nbs;to&nbs;overestimation&nbs;of&nbs;the&nbs;hytic
acid&nbs;content.3,4
Given&nbs;the&nbs;lexities&nbs;of&nbs;the&nbs;urification&nbs;and&nbs;measurement&nbs;of&nbs;hytic
acid&nbs;searate&nbs;from&nbs;lower&nbs;myo-inositol&nbs;hoshate&nbs;forms,&nbs;Megazyme
has&nbs;develoed&nbs;a&nbs;simle,&nbs;quantitative&nbs;method&nbs;(K-HYT)&nbs;to&nbs;measure
total&nbs;“available&nbs;hoshorus”&nbs;released&nbs;from&nbs;food&nbs;and&nbs;feed&nbs;samles&nbs;that
is&nbs;amenable&nbs;to&nbs;high&nbs;numbers&nbs;of&nbs;samles&nbs;and&nbs;does&nbs;not&nbs;require&nbs;tedious
anion-exchange&nbs;urification.&nbs;This&nbs;method&nbs;involves&nbs;acid&nbs;extraction&nbs;of
inositol&nbs;hoshates&nbs;followed&nbs;by&nbs;treatment&nbs;with&nbs;a&nbs;hytase&nbs;that&nbs;is
secific&nbs;for&nbs;hytic&nbs;acid&nbs;(I6)&nbs;and&nbs;the&nbs;lower&nbs;myo-inositol&nbs;hoshate
forms&nbs;(i.e.&nbs;I2,&nbs;I3,&nbs;I4,&nbs;I5).&nbs;Subsequent&nbs;treatment&nbs;with&nbs;alkaline
hoshatase&nbs;ensures&nbs;the&nbs;release&nbs;of&nbs;the&nbs;final&nbs;hoshate&nbs;from&nbs;myoinositol
hoshate&nbs;(I1)&nbs;which&nbs;is&nbs;relatively&nbs;resistant&nbs;to&nbs;the&nbs;action&nbs;of
hytase.&nbs;The&nbs;total&nbs;hoshate&nbs;released&nbs;is&nbs;measured&nbs;using&nbs;a&nbs;modified
colorimetric&nbs;method&nbs;and&nbs;given&nbs;as&nbs;ams&nbs;of&nbs;hoshorus&nbs;er&nbs;100&nbs;g&nbs;of
samle&nbs;material.5,6
1
Alkaline&nbs;hoshatase&nbs;will&nbs;also&nbs;release&nbs;hoshate&nbs;from
monohoshate&nbs;esters&nbs;other&nbs;than&nbs;myo-inositol&nbs;hoshate,&nbs;however
analyses&nbs;of&nbs;a&nbs;broad&nbs;sectrum&nbs;of&nbs;samles&nbs;using&nbs;K-HYT&nbs;indicated&nbs;that
hoshorus&nbs;released&nbs;from&nbs;monohoshate&nbs;esters&nbs;other&nbs;than&nbs;myoinositol
hoshate&nbs;is&nbs;small.
产品货号：K-HYT&nbs;
规格：50&nbs;
产地：爱尔兰&nbs;
数：&nbs;
英文名：hytic&nbs;Acid/Total&nbs;hoshorus&nbs;Assay&nbs;Kit
保存条件：4-6摄氏度
保期：1年以上
外观四个玻璃小瓶和两个60毫升聚丙烯瓶
比重&nbs;
在水中的溶解度易溶
H值&nbs;7.0
气味&nbs;无
粉末和液体形式
性&nbs;&nbs;十年或以上(冷冻机)
成分:
名称&nbs;比例
钠缓冲液60毫升&nbs;一小瓶（聚丙烯）
甘氨酸缓冲液&nbs;一瓶（聚丙烯）
植酸酶悬浮液&nbs;一个七毫升小瓶（玻璃）
碱性磷酸酶悬浮液&nbs;一个七毫升小瓶（玻璃）
磷标准溶液25毫升&nbs;一小瓶（玻璃）
燕麦粉的液&nbs;一毫升小瓶（玻璃）
试剂盒：
试剂盒适执行50个化验从
包含检测方法，另：
瓶1：钠缓冲液（25毫升，200毫米，H值5.5）和
叠氮化钠（0.02％瓦/视频作为剂）。
在4&nbs;°&nbs;C的“2年
瓶2：植酸酶暂停（1.2毫升，12000单位/毫升）。
在4&nbs;°&nbs;C的“2年
瓶3：甘氨酸缓冲液（25毫升，400毫米，H值10.4），化镁
（4毫米），锌（0.4毫米）和叠氮化钠
（0.02％瓦/&nbs;5）作为剂。
在4&nbs;°&nbs;C的“2年
瓶4：暂停碱性磷酸酶（1.2毫升，80单位/毫升）。
在4&nbs;°&nbs;C的“2年
瓶5：磷标准溶液（24毫升，50微克/毫升）。
和叠氮化钠（0.02％瓦/视频作为剂）。
在4&nbs;°&nbs;C的“2年
瓶6：燕麦粉粉（5克，磷含，
见瓶）。
“在室温下5年。
hytic&nbs;Acid&nbs;/&nbs;Total&nbs;hoshorus
A&nbs;simle&nbs;method&nbs;for&nbs;the&nbs;measurement&nbs;of&nbs;hytic&nbs;acid&nbs;/&nbs;total&nbs;hoshorus&nbs;in&nbs;food&nbs;and&nbs;feed&nbs;samles.&nbs;This&nbs;method&nbs;does&nbs;not&nbs;require&nbs;urification&nbs;of&nbs;hytic&nbs;acid&nbs;via&nbs;anion-exchange&nbs;chromatoahy&nbs;making&nbs;it&nbs;amenable&nbs;to&nbs;high&nbs;numbers&nbs;of&nbs;samles.
Catalogue&nbs;Number:&nbs;K-HYT
Content:&nbs;50&nbs;assays
Aearance&nbs;Four&nbs;glass&nbs;vials&nbs;and&nbs;two&nbs;60&nbs;mL&nbs;olyroylene&nbs;containers.
Secific&nbs;avity&nbs;Not&nbs;alicable
Solubility&nbs;in&nbs;Water&nbs;Readily&nbs;soluble.
H&nbs;Value&nbs;7.0
Odour&nbs;none
Form&nbs;owders&nbs;and&nbs;liquids
Stability&nbs;stable&nbs;in&nbs;a&nbs;freezer&nbs;for&nbs;ten&nbs;or&nbs;more&nbs;years
Inedients&nbs;Name&nbs;CAS&nbs;roortion
Sodium&nbs;acetate&nbs;buffer&nbs;One&nbs;60&nbs;ml&nbs;vial&nbs;(olyroylene)
Glycine&nbs;Buffer&nbs;One&nbs;container&nbs;(olyroylene)
hytase&nbs;susension&nbs;One&nbs;7&nbs;ml&nbs;vial&nbs;(glass)
Alkaline&nbs;hoshatase&nbs;susension&nbs;One&nbs;7&nbs;ml&nbs;vial&nbs;(glass)
hoshorus&nbs;standard&nbs;solution&nbs;One&nbs;25&nbs;ml&nbs;vial&nbs;(glass)
Oat&nbs;flour&nbs;control&nbs;One&nbs;12&nbs;ml&nbs;vial&nbs;(glass)
KITS:
Kits&nbs;suitable&nbs;for&nbs;erforming&nbs;50&nbs;assays&nbs;are&nbs;available.The
kits&nbs;contain&nbs;the&nbs;full&nbs;assay&nbs;method&nbs;lus:
Bottle&nbs;1:&nbs;Sodium&nbs;acetate&nbs;buffer&nbs;(25&nbs;mL,&nbs;200&nbs;mM,&nbs;H&nbs;5.5)&nbs;and
sodium&nbs;azide&nbs;(0.02&nbs;%&nbs;w/v)&nbs;as&nbs;a&nbs;reservative.
Stable&nbs;for&nbs;>&nbs;2&nbs;years&nbs;at&nbs;4°C.
Bottle&nbs;2:&nbs;hytase&nbs;susension&nbs;(1.2&nbs;mL,&nbs;12,000&nbs;U/mL).
Stable&nbs;for&nbs;>&nbs;2&nbs;years&nbs;at&nbs;4°C.
Bottle&nbs;3:&nbs;Glycine&nbs;buffer&nbs;(25&nbs;mL,&nbs;400&nbs;mM,&nbs;H&nbs;10.4),&nbs;lus&nbs;MgCl2
(4&nbs;mM),&nbs;ZnSO4&nbs;(0.4&nbs;mM)&nbs;and&nbs;sodium&nbs;azide
(0.02&nbs;%&nbs;w/v)&nbs;as&nbs;a&nbs;reservative.
Stable&nbs;for&nbs;>&nbs;2&nbs;years&nbs;at&nbs;4°C.
Bottle&nbs;4:&nbs;Alkaline&nbs;hoshatase&nbs;susension&nbs;(1.2&nbs;mL,&nbs;80&nbs;U/mL).
Stable&nbs;for&nbs;>&nbs;2&nbs;years&nbs;at&nbs;4°C.
Bottle&nbs;5:&nbs;hoshorus&nbs;standard&nbs;solution&nbs;(24&nbs;mL,&nbs;50&nbs;μg/mL).
and&nbs;sodium&nbs;azide&nbs;(0.02&nbs;%&nbs;w/v)&nbs;as&nbs;a&nbs;reservative.
Stable&nbs;for&nbs;>&nbs;2&nbs;years&nbs;at&nbs;4°C.
Bottle&nbs;6:&nbs;Oat&nbs;flour&nbs;control&nbs;owder&nbs;(5&nbs;g;&nbs;hoshorus&nbs;content,
see&nbs;bottle).
Stable&nbs;for&nbs;>&nbs;5&nbs;years&nbs;at&nbs;room&nbs;temerature.
INTRODUCTION:
hytic&nbs;acid&nbs;(hytate;&nbs;myo-inositol&nbs;1,2,3,4,5,6,&nbs;hexakishoshate)&nbs;is&nbs;the
rimary&nbs;source&nbs;of&nbs;inositol&nbs;and&nbs;storage&nbs;hoshorus&nbs;in&nbs;lant&nbs;seeds
contributing&nbs;~70&nbs;%&nbs;of&nbs;total&nbs;hoshorus.&nbs;The&nbs;abundance&nbs;of&nbs;hytic&nbs;acid
in&nbs;cereal&nbs;ains&nbs;is&nbs;a&nbs;concern&nbs;in&nbs;the&nbs;foods&nbs;and&nbs;animal&nbs;feeds&nbs;industries
because&nbs;the&nbs;hoshorus&nbs;in&nbs;this&nbs;form&nbs;is&nbs;unavailable&nbs;to&nbs;monogastric
animals&nbs;due&nbs;to&nbs;a&nbs;lack&nbs;of&nbs;endogenous&nbs;hytases;&nbs;enzymes&nbs;secific&nbs;for
the&nbs;dehoshorylation&nbs;of&nbs;hytic&nbs;acid.&nbs;In&nbs;addition,&nbs;the&nbs;strong&nbs;chelating
characteristic&nbs;of&nbs;hytic&nbs;acid&nbs;reduces&nbs;the&nbs;bioavailability&nbs;of&nbs;other
essential&nbs;dietary&nbs;nutrients&nbs;such&nbs;as&nbs;minerals&nbs;(e.g.&nbs;Ca2+,&nbs;Zn2+,&nbs;Mg2+,
Mn2+,&nbs;Fe2+/3+),&nbs;roteins&nbs;and&nbs;amino&nbs;acids.1&nbs;High&nbs;hytic&nbs;acid&nbs;content
feeds&nbs;are&nbs;generally&nbs;sulemented&nbs;with&nbs;inorganic&nbs;hoshate,&nbs;however
this&nbs;causes&nbs;increased&nbs;faecal&nbs;hoshate&nbs;levels&nbs;and&nbs;subsequent
eutrohication&nbs;of&nbs;waterways.&nbs;Alternatively,&nbs;sulementation&nbs;with
mercial&nbs;hytases&nbs;is&nbs;being&nbs;increasingly&nbs;oular&nbs;and&nbs;reduces&nbs;the
requirement&nbs;for&nbs;inorganic&nbs;hoshate&nbs;sulementation&nbs;as&nbs;well&nbs;as&nbs;the
associated&nbs;environmental&nbs;issues.
Currently,&nbs;there&nbs;is&nbs;no&nbs;mercially&nbs;available,&nbs;simle,&nbs;quantitative
method&nbs;for&nbs;hytic&nbs;acid&nbs;and&nbs;while&nbs;such&nbs;measurement&nbs;is&nbs;relatively
lex&nbs;the&nbs;generally&nbs;acceted&nbs;AOAC&nbs;Method&nbs;986.11&nbs;has
limitations.2&nbs;For&nbs;each&nbs;individual&nbs;analysis&nbs;the&nbs;method&nbs;requires
cumbersome&nbs;anion-exchange&nbs;urification&nbs;and&nbs;a&nbs;major&nbs;inherent
assumtion&nbs;here&nbs;is&nbs;that&nbs;only&nbs;hytic&nbs;acid&nbs;is&nbs;urified.&nbs;While&nbs;this
assumtion&nbs;is&nbs;viable&nbs;for&nbs;non-rocessed&nbs;ains&nbs;for&nbs;which&nbs;hytic&nbs;acid
rises&nbs;at&nbs;least&nbs;97&nbs;%&nbs;of&nbs;total&nbs;inositol&nbs;hoshates,&nbs;it&nbs;is&nbs;not&nbs;viable&nbs;for
rocessed&nbs;foods&nbs;and&nbs;feeds&nbs;which&nbs;can&nbs;contain&nbs;higher&nbs;levels&nbs;of&nbs;some
lower&nbs;myo-inositol&nbs;hoshate&nbs;forms&nbs;(i.e.&nbs;I3,&nbs;I4,&nbs;I5)&nbs;that&nbs;would&nbs;coelute
with&nbs;hytic&nbs;acid&nbs;and&nbs;contribute&nbs;to&nbs;overestimation&nbs;of&nbs;the&nbs;hytic
acid&nbs;content.3,4
Given&nbs;the&nbs;lexities&nbs;of&nbs;the&nbs;urification&nbs;and&nbs;measurement&nbs;of&nbs;hytic
acid&nbs;searate&nbs;from&nbs;lower&nbs;myo-inositol&nbs;hoshate&nbs;forms,&nbs;Megazyme
has&nbs;develoed&nbs;a&nbs;simle,&nbs;quantitative&nbs;method&nbs;(K-HYT)&nbs;to&nbs;measure
total&nbs;“available&nbs;hoshorus”&nbs;released&nbs;from&nbs;food&nbs;and&nbs;feed&nbs;samles&nbs;that
is&nbs;amenable&nbs;to&nbs;high&nbs;numbers&nbs;of&nbs;samles&nbs;and&nbs;does&nbs;not&nbs;require&nbs;tedious
anion-exchange&nbs;urification.&nbs;This&nbs;method&nbs;involves&nbs;acid&nbs;extraction&nbs;of
inositol&nbs;hoshates&nbs;followed&nbs;by&nbs;treatment&nbs;with&nbs;a&nbs;hytase&nbs;that&nbs;is
secific&nbs;for&nbs;hytic&nbs;acid&nbs;(I6)&nbs;and&nbs;the&nbs;lower&nbs;myo-inositol&nbs;hoshate
forms&nbs;(i.e.&nbs;I2,&nbs;I3,&nbs;I4,&nbs;I5).&nbs;Subsequent&nbs;treatment&nbs;with&nbs;alkaline
hoshatase&nbs;ensures&nbs;the&nbs;release&nbs;of&nbs;the&nbs;final&nbs;hoshate&nbs;from&nbs;myoinositol
hoshate&nbs;(I1)&nbs;which&nbs;is&nbs;relatively&nbs;resistant&nbs;to&nbs;the&nbs;action&nbs;of
hytase.&nbs;The&nbs;total&nbs;hoshate&nbs;released&nbs;is&nbs;measured&nbs;using&nbs;a&nbs;modified
colorimetric&nbs;method&nbs;and&nbs;given&nbs;as&nbs;ams&nbs;of&nbs;hoshorus&nbs;er&nbs;100&nbs;g&nbs;of
samle&nbs;material.5,6
1
Alkaline&nbs;hoshatase&nbs;will&nbs;also&nbs;release&nbs;hoshate&nbs;from
monohoshate&nbs;esters&nbs;other&nbs;than&nbs;myo-inositol&nbs;hoshate,&nbs;however
analyses&nbs;of&nbs;a&nbs;broad&nbs;sectrum&nbs;of&nbs;samles&nbs;using&nbs;K-HYT&nbs;indicated&nbs;that
hoshorus&nbs;released&nbs;from&nbs;monohoshate&nbs;esters&nbs;other&nbs;than&nbs;myoinositol
hoshate&nbs;is&nbs;small.
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