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CHELATING SEPHAROSE FAST LOW 17057502 Cytiva/思拓凡

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  • 是否进口
  • 氨基酸分类
  • GE品牌
北京 顺义区 7天内发货 100盒
北京优尼康生物科技有限公司 1年
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产品详情

Chelating Sepharose Fast Flow immobilized metal affinity chromatography resin Chelating Sepharose Fast Flow is an uncharged IMAC chromatography resin for purification of proteins and peptides with affinity for metal ions. Ready to charge with your metal ion of choice for optimized selectivity. Based on well-established Sepharose Fast Flow resins. High chemical stability enables proven CIP and sanitization protocols. Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool. Fulfills industrial demands for security of supply, robust performance, and regulatory support. Chelating resin for flexible and reliable IMAC purification Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic, which comes immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms. The highly crosslinked agarose resin enables high flow rates and high productivity. This immobilized metal affinity chromatography (IMAC) resin is designed to purify a wide range of proteins. It is supplied free of metal ions (uncharged), allowing the user to charge it with the most appropriate metal ion for purification of a target protein. Streamlined scale up Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward. Successful use – research scale Chelating Sepharose Fast Flow is an established tool for a range of research applications. For instance, this metal chelate affinity chromatography resin has been used to purify his-tagged proteins for the following purposes: Map the spatial structure of the dimeric transmembrane domain of the growth factor receptor ErbB2 Identify distinct PIases with proven enzyme activity in E. coli Compatible empty columns – research scale Cytiva's solutions include a variety of empty columns for research scale. Chelating Sepharose Fast Flow is compatible with two of these lines, Tricorn and XK columns. Compatible empty columns – bioprocess scale For process-scale applications, Cytiva recommends AxiChrom columns. Chelating Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow. Prepacked Chelating Sepharose Fast Flow columns – research scale This resin is available as HiTrap columns for use with a syringe, a pump, or a chromatography system. Prepacked Chelating Sepharose Fast Flow columns – bioprocess scale Prepacked ReadyToProcess columns for bioprocess applications are available on request, eliminating the need for column packing and cleaning validation.

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